MRE11 Rabbit Polyclonal Antibody

CAT#: TA319245

Rabbit polyclonal anti-Mre11 antibody

Product Images

ChIP using Mre11 (S. cerevisiae) antibody. Chromatin was immunoprecipitated with the antibody at the stated dilutions. Immunocomplexes were captured using polyacrylamide bead linked secondary antibodies. The resultant immunoprecipitate was probed by multiplex PCR, using primers 20 bp from the MAT locus double strand break (lower arrow) and 67 kb from the break (upper band, control locus).

Specifications

Product Data

• Applications: IP
• Recommended Dilution: ELISA: 1:10,000 - 1:50,000, WB: 1:500- 1:2,000
• Reactivities: Saccharomyces cerevisiae
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 578-590 of Saccharomyces cerevisiae (baker's yeast) Mre11 protein.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: MRE11 homolog A, double strand break repair nuclease
• Database Link:
  NP_005581
  Entrez Gene 4361 Human
  UniProt ID P49959
• Synonyms: ATLD; HNGS1; MRE11; MRE11B
• Note: Mre11 (also known as double-strand break repair protein MRE11) is a subunit of a complex with Rad50 and Xrs2 (RMX complex) that functions in repair of DNA double-strand breaks and in telomere stability. Mre11 possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity that appears to be required for RMX function. This nuclear protein is widely conserved and is also involved in meiotic double strand break processing.

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Homologous recombination, Non-homologous end-joining