NFAT4 (NFATC3) Rabbit Polyclonal Antibody

CAT#: TA316498

Rabbit polyclonal anti-NFAT4 (Ser165) antibody (Phospho-specific)

Product Images

Western blot - NFAT4 (phospho S165) antibody. All lanes : Anti-NFAT4 (phospho S165) antibody at 1/500 dilution. Lane 1 : HeLa cells treated with calcium (40nM, 30 mins). Lane 2 : HeLa cells treated with calcium (40nM, 30 mins) with immunizing phosphopeptide. Predicted band size : 116 kDa. Observed band size : 116 kDa.

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  Immunofluorescence analysis of HeLa cells, using NFAT4 (Phospho-Ser165) antibody. Left: without immunizing phosphopeptide; Right: with immunizing phosphopeptide.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1:500~1:3000, IF: 1:100~1:500, ELISA: 1:1000
• Reactivities: Human
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: nuclear factor of activated T-cells 3
• Database Link:
  NP_004546
  Entrez Gene 4775 Human
  UniProt ID Q12968
• Synonyms: NFAT4; NFATX
• Note: NFAT4 (Phospho-Ser165) antibody detects endogenous levels of NFAT4 only when phosphorylated at serine 165.

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Axon guidance, B cell receptor signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway