FOXO1 Rabbit Polyclonal Antibody

CAT#: TA309983

Rabbit anti-FKHR (Phospho-Ser319) polyclonal antibody(Phospho-specific)

Product Images

Western blot analysis of extracts from 293 cells (10% serum-treated, 15min) and HeLa cells (EGF-treated, 200ng/ml, 15min), using FKHR antibody (Lanes 1, 2 and 3) and FKHR (phospho-Ser319) antibody (Lanes 4 and 5).

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  Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using FKHR (phospho-Ser319) antibody.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:500~1:1000, IHC: 1:50~1:100
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from humanFKHR around the phosphorylation site of serine 319 (T-S-SP-N-A).
• Formulation: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: forkhead box O1
• Database Link:
  NP_002006
  Entrez Gene 56458 MouseEntrez Gene 84482 RatEntrez Gene 2308 Human
  UniProt ID Q12778
• Synonyms: FKH1; FKHR; FOXO1A

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Insulin signaling pathway, Pathways in cancer, Prostate cancer