alpha Tubulin (TUBA1A) Rat Monoclonal Antibody [Clone ID: YL1/2]

CAT#: TA309922

Rat monoclonal anti-TUBA1A(alpha Tubulin ) antibody, clone YL1/2, Loading control

Product Images

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) TA309922 - Tubulin YL1/2 antibody was tested in HeLa cells with Dylight 550 (red). Nuclei were counterstained with DAPI (blue).

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  Flow Cytometry: alpha Tubulin Antibody (YL1/2) TA309922 - An intracellular stain was performed on PC12 cells with Tubulin [YL1/2] Antibody TA309922AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

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  Western Blot: alpha Tubulin Antibody (YL1/2) TA309922 - Western Blot analysis of whole cell lysates of HeLa, NIH-3T3 and PC-12 cell lines using Tubulin antibody (clone YL1/2). Alpha tubulin molecular weight: 50 kDa.

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  Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) TA309922 - IF Confocal analysis of NIH/3T3 cells using Tubulin antibody (TA309922, 1:5). An Alexa Fluor 488-conjugated Goat to rat IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).

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  Flow Cytometry: alpha Tubulin Antibody (YL1/2) TA309922 - An intracellular stain was performed on SH-SY5Y cells with alpha Tubulin (YL1/2) Antibody [TA309922G] (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488.

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  Western Blot: alpha Tubulin Antibody (YL1/2) TA309922 - Western blot analysis of alpha Tubulin (molecular weight: 50 kDa) expression in 1) HeLa, 2) NTERA-2, 3) A431, 4) HepG2, 5) MCF7, 6) NIH-3T3, 7) PC-12 and 8) Cos 7 whole cell lysates using TA309922.

• Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) TA309922 - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Tubulin (YL1/2) at a 1:200 dilution overnight at 4C and detected with an anti-rat Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

• Flow Cytometry: alpha Tubulin Antibody (YL1/2) TA309922 - An intracellular stain was performed on NIH3T3 cells with Tubulin [YL1/2] Antibody TA309922AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

• Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) TA309922 - HeLa cells were fixed and permeabilized for 10 minutes with -20C MeOH. The cells were incubated with alpha Tubulin Antibody [YL1/2] (TA309922) at 1ug/ml overnight at 4C and detected with an anti-rat DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

• Flow Cytometry: alpha Tubulin Antibody (YL1/2) TA309922 - Analysis of Alexa Fluor (R) 647 conjugate of TA309922. An intracellular stain was performed on HeLa cells with Tubulin antibody (YL1/2) TA309922AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

• Western Blot: alpha Tubulin Antibody (YL1/2) TA309922 - Total protein from human HeLa, mouse 3T3, rat PC12 and African green monkey Cos7 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with a 1:6000 dilution of anti-Tubulin (YL 1/2) in 1% non-fat milk in TBST and detected with an anti-rat HRP secondary antibody using chemiluminescence. Alpha tubulin molecular weight: 50 kDa.

• Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) TA309922 - Rat FR cells were fixed and permeabilized for 10 minutes with -20C MeOH. The cells were incubated with alpha Tubulin Antibody [YL1/2] (TA309922) at 1ug/ml overnight at 4C and detected with an anti-rat DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

• Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) TA309922 - NIH3T3 cells were fixed and permeabilized for 10 minutes with -20C MeOH. The cells were incubated with alpha Tubulin Antibody [YL1/2] (TA309922) at 1ug/ml overnight at 4C and detected with an anti-rat DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

• Immunohistochemistry-Paraffin: alpha Tubulin Antibody (YL1/2) TA309922 - Tubulin antibody was tested in human breast cancer xenograft using DAB with hematoxylin counterstain.

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Specifications

Product Data

• Clone Name: YL1/2
• Applications: ELISA, FC, ICC/IF, IHC, IP, WB
• Recommended Dilution: ELISA: 1:100-1:1000, Immunohistochemistry: 1:200, Immunoprecipitation: 1:10-1:500, Western Blot: 1:5000-1:10000, Immunohistochemistry-Frozen: 1:200, Radioimmunoassay, Immunocytochemistry/ Immunofluorescence: 1:1000-1:10000, Functional, Immunohistochemistry-Paraffin: 1:200, Flow Cytometry: 2-5 ug/0.1x10^6 cells
• Reactivities: Human, Mammalian, Mouse, Rat, Yeast
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length native protein (purified) (S. cerevisiae).
• Formulation: PBS, [pH7.4] and 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: tubulin alpha 1a
• Database Link:
  NP_006000
  Entrez Gene 22142 MouseEntrez Gene 64158 RatEntrez Gene 7846 Human
  UniProt ID Q71U36
• Synonyms: B-ALPHA-1; LIS3; TUBA3

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Gap junction, Pathogenic Escherichia coli infection