14-3-3 beta (YWHAB) Rabbit Polyclonal Antibody

CAT#: TA308715

Rabbit Polyclonal antibody to 14-3-3 beta (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide)

Product Images

Sample (20 ug of whole cell lysate). A: mouse brain. 12% SDS PAGE. TA308715 diluted at 1:10000.

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  Sample (30 ug of whole cell lysate). A: A431. B: H1299. C: Hela. D: Hep G2. E: Molt-4. F: Raji. 12% SDS PAGE. TA308715 diluted at 1:10000.

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  Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using 14-3-3 beta (TA308715) antibody at 1:500 dilution.

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  Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 14-3-3 beta (TA308715) antibody at 1:200 dilution.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:5000-1:20000
• Reactivities: Human (Predicted: Bovine, Rhesus Monkey)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 171 of 14-3-3 beta (Uniprot ID#P31946)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28 kDa
• Gene Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta
• Database Link:
  NP_003395
  Entrez Gene 7529 Human
  UniProt ID P31946
• Background: This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq]
• Synonyms: GW128; HEL-S-1; HS1; KCIP-1; YWHAA
• Note: Seq homology of immunogen across species: Rhesus Monkey (100%), Bovine (100%)

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Neurotrophin signaling pathway, Oocyte meiosis