Oct4 (POU5F1) Rabbit Polyclonal Antibody

CAT#: TA306913

Rabbit Polyclonal POU5F1 Antibody

Product Images

Western blot analysis of POU5F1 in mouse liver tissue lysate with POU5F1 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.

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  Immunohistochemistry of POU5F1 in rat liver tissue with POU5F1 antibody at 5 ugg/mL.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1 ug/mL, ICC: 5 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: POU5F1 antibody was raised against a 14 amino acid peptide near the carboxy terminus of human POU5F1.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: POU class 5 homeobox 1
• Database Link:
  NP_002692
  Entrez Gene 18999 MouseEntrez Gene 294562 RatEntrez Gene 5460 Human
  UniProt ID Q01860
• Background: POU5F1, also commonly known as Oct-4, is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. The role of POU5F1 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing POU5F1, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.
• Synonyms: Oct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4

Reference Data

• Protein Families: Adult stem cells, Cancer stem cells, Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency, Transcription Factors