Apg12 (ATG12) Rabbit Polyclonal Antibody

CAT#: TA306511

Rabbit Polyclonal ATG12 Antibody

Product Images

Western blot analysis of ATG12 in human brain tissue lysate with ATG12 antibody at (A) 0.5, and (B) 1 ug/ml.

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  Immunohistochemistry of ATG12 in human brain tissue with ATG12 antibody at 2.5 ug/ml.

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  Immunofluorescence of ATG12 in Human Brain cells with ATG12 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 0.5 - 1 ug/mL, ICC: 2.5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: ATG12 antibody was raised against a 16 amino acid peptide from near the amino terminus of human ATG12.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: autophagy related 12
• Database Link:
  NP_004698
  Entrez Gene 67526 MouseEntrez Gene 361321 RatEntrez Gene 9140 Human
  UniProt ID O94817
• Background: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG12, another member of the autophagy protein family, forms a conjugate with ATG5; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7. At least two isoforms of ATG12 are known to exist.
• Synonyms: APG12; APG12L; FBR93; HAPG12

Reference Data

• Protein Pathways: Regulation of autophagy, RIG-I-like receptor signaling pathway