SFRP4 Goat Polyclonal Antibody

CAT#: TA305645

Goat Anti-SFRP4 Antibody

Product Images

TA305645 (0.5 ug/ml) staining of Human Uterus lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  TA305645 (2 ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1-3 µg/ml. IHC: 2-4 µg/ml.
• Reactivities: Human (Expected from sequence similarity: Mouse, Rat, Cow, Pig)
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence TNSSCQCPHILPHQD, from the internal region of the protein sequence according to NP_003005.2.
• Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: secreted frizzled related protein 4
• Database Link:
  NP_003005
  Entrez Gene 20379 MouseEntrez Gene 89803 RatEntrez Gene 6424 Human
  UniProt ID Q6FHJ7
• Background: Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. [provided by RefSeq]
• Synonyms: FRP-4; FRPHE; sFRP-4
• Note: IHC: In paraffin embedded Human Uterus shows granular staining in the apical cytoplasm of the endometrial gland.

Reference Data

• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - Wnt Signaling pathway
• Protein Pathways: Wnt signaling pathway