PNPLA3 Goat Polyclonal Antibody

CAT#: TA303343

Goat Polyclonal Antibody against PNPLA3

Product Images

TA303343 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). This data is from a previous batch, not on sale.

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  TA303343 (0.03µg/ml) staining of Mouse Adrenal Gland lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

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  TA303343 (8µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

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  TA303343 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.

Specifications

Product Data

• Applications: IHC, PEP-ELISA, WB
• Recommended Dilution: ELISA: 1:128,000. WB: 0.01-0.03µg/ml.IHC:4-6µg/ml.
• Reactivities: Human, Mouse (Expected from sequence similarity: Rat, Dog, Pig)
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence EHDICPKVKSTN, from the internal region of the protein sequence according to NP_473429.2.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: patatin like phospholipase domain containing 3
• Database Link:
  NP_079501
  Entrez Gene 116939 MouseEntrez Gene 362972 RatEntrez Gene 609368 DogEntrez Gene 80339 Human
  UniProt ID Q9NST1
• Background: The protein encoded by this gene is a triacylglycerol lipase that mediates triacylglycerol hydrolysis in adipocytes. The encoded protein, which appears to be membrane bound, may be involved in the balance of energy usage/storage in adipocytes. [provided by RefSeq]
• Synonyms: ADPN; C22orf20; iPLA(2)epsilon

Reference Data

• Protein Pathways: Glycerolipid metabolism, Glycerophospholipid metabolism, Limonene and pinene degradation, Metabolic pathways, Phenylalanine metabolism, Tyrosine metabolism