WASP (WAS) Goat Polyclonal Antibody

CAT#: TA303004

Goat Polyclonal Antibody against WAS

Product Images

TA303004 staining (0.03ug/ml) of U937 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:128,000. WB: 0.03-0.1µg/ml.
• Reactivities: Human (Expected from sequence similarity: Mouse, Rat, Dog)
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-SPADKKRSGKKKI, from the internal region of the protein sequence according to NP_000368.1.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Wiskott-Aldrich syndrome
• Database Link:
  NP_000368
  Entrez Gene 22376 MouseEntrez Gene 317371 RatEntrez Gene 491867 DogEntrez Gene 7454 Human
  UniProt ID P42768
• Background: The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Wiskott-Aldrich syndrome is a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia, and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients. A transcript variant arising as a result of alternative promoter usage, and containing a different 5' UTR sequence, has been described, however, its full-length nature is not known. [provided by RefSeq]
• Synonyms: IMD2; SCNX; THC; THC1; WASP; WASPA

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Chemokine signaling pathway, Fc gamma R-mediated phagocytosis, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton