VPS28 Goat Polyclonal Antibody

CAT#: TA302924

Goat Polyclonal Antibody against VPS28

Product Images

TA302924 staining (1ug/ml) of Human Testis lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

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  TA302924 (10ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1:1,000. WB: 1-3µg/ml. IHC: 10µg/ml.
• Reactivities: Human (Expected from sequence similarity: Mouse, Rat, Dog, Pig)
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-ESAYNAFNRFLHA, from the C Terminus of the protein sequence according to NP_057292.1.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: VPS28, ESCRT-I subunit
• Database Link:
  NP_057292
  Entrez Gene 66914 MouseEntrez Gene 300052 RatEntrez Gene 607677 DogEntrez Gene 51160 Human
  UniProt ID Q9UK41
• Background: This gene encodes a protein involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. The encoded protein is one of the three subunits of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. The two other subunits of ESCRT-I are vesicular protein sorting 23, also known as tumor susceptibility gene 101 (TSG101), and vesicular protein sorting 37. Two alternative transcripts encoding different isoforms have been described. Additional alternative transcripts may exist but the proteins encoded by these transcripts have not been verified experimentally.
• Synonyms: MGC60323

Reference Data

• Protein Pathways: Endocytosis