NFIL3 Goat Polyclonal Antibody

CAT#: TA302736

Goat Polyclonal Antibody against NFIL3

Product Images

TA302736 (2ug/ml) of Daudi cell lysate (RIPA buffer, 20ug total protein per lane). Detected by chemiluminescence.

• 

  Flow cytometric analysis of paraformaldehyde fixed Human peripheral blood mononuclear cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution: WB: 1-3µg/ml.
• Reactivities: Human (Expected from sequence similarity: Mouse, Rat)
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-RLIATQPISASDSG, from the C Terminus of the protein sequence according to NP_005375.2.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: nuclear factor, interleukin 3 regulated
• Database Link:
  NP_005375
  Entrez Gene 18030 MouseEntrez Gene 114519 RatEntrez Gene 4783 Human
  UniProt ID Q16649
• Background: Expression of interleukin-3 (IL3; MIM 147740) is restricted to activated T cells, natural killer (NK) cells, and mast cell lines. Transcription initiation depends on the activating capacity of specific protein factors, such as NFIL3, that bind to regulatory regions of the gene, usually upstream of the transcription start site (Zhang et al., 1995 [PubMed 7565758]). [supplied by OMIM]
• Synonyms: E4BP4; IL3BP1; NF-IL3A; NFIL3A

Reference Data

• Protein Families: Transcription Factors