VPS35 Goat Polyclonal Antibody

CAT#: TA302699

Goat Polyclonal Antibody against VPS35 / MEM3

Product Images

TA302699 (1ug/ml) staining of Human Brain (Cerebellum) (A) and Rat Brain (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  TA302699 (5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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  TA302699 (5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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  TA302699 staining of PFA-fixed and saponin permeabilized HEK293 and detected with FITC in confocal microscopy. Data obtained from Aarhus University Denmark, with a previous batch.

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  TA302699 staining of PFA-fixed and saponin permeabilized SHSY5Y and detected with FITC in confocal microscopy. Data obtained, with a previous batch, from Dr. M. Schallburg Nielsen, Aarhus University Denmark.

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Specifications

Product Data

• Applications: IF, IHC, PEP-ELISA, WB
• Recommended Dilution: ELISA: 1:128000. WB: 1-3µg/ml. IHC:5µg/ml
• Reactivities: Human (Expected from sequence similarity: Mouse, Rat, Cow)
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-SPESEGPIYEGLIL, from the C Terminus of the protein sequence according to NP_060676.2.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 91.5 kDa
• Gene Name: VPS35 retromer complex component
• Database Link:
  NP_060676
  Entrez Gene 25479 RatEntrez Gene 65114 MouseEntrez Gene 55737 Human
  UniProt ID Q96QK1
• Background: This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: MEM3; PARK17
• Note: In paraffin embedded Human Testis, shows positive staining in Leydig cells, and cytoplasmic staining (dot-like) of some epithelial cells of the epididymis and of the rete testis. Data obtained with a previous batch.