Hsc70 (HSPA8) Goat Polyclonal Antibody

CAT#: TA302666

Goat Polyclonal Antibody against HSPA8 (Isoform 1)

Product Images

TA302666 (0.03µg/ml) staining of HepG2 (A) and NIH3T3 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

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  TA302666 (5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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  TA302666 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Peptide-ELISA: 1:4,000.
  WB: 1-3µg/ml.
  IF: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10µg/ml
  IHC: Paraffin embedded Human Prostate. Recommended concentration: 5µg/ml.
• Reactivities: Human, Mouse (Expected from sequence similarity: Rat, Dog)
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-EKLQGKINDEDKQK, from the internal region of the protein sequence according to NP_006588.1.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 70.7 kDa
• Gene Name: heat shock protein family A (Hsp70) member 8
• Database Link:
  NP_006588
  Entrez Gene 15481 MouseEntrez Gene 24468 RatEntrez Gene 479406 DogEntrez Gene 3312 Human
  UniProt ID P11142
• Background: The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date. [provided by RefSeq]
• Synonyms: HEL-33; HEL-S-72p; HSC54; HSC70; HSC71; HSP71; HSP73; HSPA10; LAP-1; LAP1; NIP71

Reference Data

• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Antigen processing and presentation, Endocytosis, MAPK signaling pathway, Spliceosome