Cofilin 2 (CFL2) Goat Polyclonal Antibody

CAT#: TA302652

Goat Polyclonal Antibody against Cofilin 2 (muscle)

Product Images

TA302652 (0.003ug/ml) staining of human muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:128,000. WB: 0.01-0.03µg/ml.
• Reactivities: Human, Mouse, Rat (Expected from sequence similarity: Dog, Pig)
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-DIKDRSTLGEK, from the C Terminus of the protein sequence according to NP_068733.1; NP_619579.1.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 18.6 kDa
• Gene Name: cofilin 2
• Database Link:
  NP_619579
  Entrez Gene 12632 MouseEntrez Gene 366624 RatEntrez Gene 490649 DogEntrez Gene 1073 Human
  UniProt ID Q9Y281
• Background: Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. (Gillett et al., 1996 [PubMed 8800436]). Cofilin-2 is a member of the AC group of proteins that also includes cofilin-1 (CFL1) and destrin (DSTN; MIM 609114), all of which regulate actin-filament dynamics (Bamburg et al., 1999 [PubMed 10461190]; Maciver and Hussey, 2002). The CFL2 gene encodes a skeletal muscle-specific isoform (Vartiainen et al., 2002 [PubMed 11809832]) localized to the thin filaments, where it exerts its effect on actin, in part through interactions with tropomyosins (Ono and Ono, 2002 [PubMed 11901171]). [supplied by OMIM]
• Synonyms: NEM7

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton