Strep-tag II Rabbit Polyclonal Antibody

CAT#: TA100024

Rabbit Polyclonal Anti-StrepII-tag Antibody

Product Images

Western blot analysis of 50ng of StrepII-tagged recombinant protein with StrepII antibody at 1 ug/mL in (A) the absence or (B) the presence of blocking peptide.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1 ug/mL
• Reactivities: Strep-tag II
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: StrepII-tag antibody was raised against the amino acid sequence "gggWSHPQFEK".
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Background: StrepII-tag Antibody: Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag is a nine amino acid peptide with high specificity and affinity towards streptavidin. The addition of this tag to the C-terminus of recombinant proteins allowed the simple purification of protein by use of affinity columns. It was re-engineered to allow it to also placed at the N-terminus of recombinant proteins.