Von Willebrand Factor (VWF) Sheep Polyclonal Antibody

CAT#: SP1313P

Von Willebrand Factor (VWF) sheep polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, FC, ID, IF, IHC
• Recommended Dilution: Immunodiffusion: Neat.
  ELISA.
  Flow Cytometry.
  Immunofluorescence.
  Immunohistochemistry on Frozen Sections: The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation is recommended for frozen sections.
  Recommended Positive Control: Tonsil.
  Does not work on paraffin sections!.
• Reactivities: Human
• Host: Sheep
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Purified Human von Willebrand factor.
• Specificity: This antibody recognizes Human Willebrand factor VIII. Other species not tested.
• Formulation: Glycine buffered saline pH 7.4, 0.01% Benzamidine, 0.1% EACA and 1mM EDTA
  State: Purified
  State: Liquid purified IgG fraction from Serum
  Preservative: 0.09% Sodium Azide
• Purification: Ion Exchange Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: von Willebrand factor
• Database Link:
  Entrez Gene 7450 Human
  UniProt ID P04275
• Background: Von Willebrand Factor (VWF) was previously known as Factor VIII related antigenis. VWF is synthesized exclusively by endothelial cells and megakaryocytes, and stored in the intracellular granules or constitutively secreted into plasma. This glycoprotein functions as both an antihemophilic factor carrier and a platelet vessel wall mediator in the blood coagulation system. Important in the maintenance of homeostasis, it participates in platelet vessel wall interactions by forming a noncovalent complex with coagulation factor VIII at the site of vascular injury. The Von Willebrand factor has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. Mutations in this gene or deficiencies in this protein result in Von Willebrand's disease. VWD is characterized by frequent bleeding (gingival, minor skin quantitative lacerations, menorrhagia, etc.).
• Synonyms: vWF, von Willebrand antigen 2, von Willebrand antigen II, F8VWF, Factor VIII Related Antigen