PIN1 Mouse Monoclonal Antibody [Clone ID: 3G8]

CAT#: SM6035S

PIN1 mouse monoclonal antibody, clone 3G8, Purified

Product Images

Western Blot: Cell lysates of 293T, HeLa and Jurkat (each 50ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Pin1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Product Data

• Clone Name: 3G8
• Applications: ELISA, FC, WB
• Recommended Dilution: ELISA.
  Western blot: 1/500-1/1,000 (Recommended starting dilution is 1/500).
  Flow Cytometry.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Human Pin1 (1-163 aa) purified from E. coli
• Specificity: This antibody is specific for Human Pin1.
  Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: peptidylprolyl cis/trans isomerase, NIMA-interacting 1
• Database Link:
  Entrez Gene 5300 Human
  UniProt ID Q13526
• Background: Human Pin1 is a peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and essential for cell cycle regulation. Pin1 is nuclear PPIase containing a WW protein interaction domain, and is structurally and functionally related to Ess1/Ptf1, an essential protein in budding yeast. PPIase activity is necessary for Ess1/Pin1 function in yeast. Pin1 is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Substrates of Pin1 include the mitotic regulators (Cdc25 phosphatase and NIMA ,PLK I, Wee, and Myt1 kinases), several transcription factors like beta-Catenin, c-Jun, and the tumor suppressor protein p53 , and some specific proteins like the RNA Pol II, the cytoskeleton protein tau, and the G1/S protein Cyclin D1.
• Synonyms: PIN-1