Alpha B Crystallin (CRYAB) Mouse Monoclonal Antibody [Clone ID: 2E8]

CAT#: SM6011

Alpha B Crystallin (CRYAB) mouse monoclonal antibody, clone 2E8, Purified

Product Images

Western blot analysis: Recombinant a-crystallin A (aA) and the extract of mouse eye (ME) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human a-crystallin B antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Product Data

• Clone Name: 2E8
• Applications: ELISA, FC, WB
• Recommended Dilution: ELISA.
  Western blot: 1/500-1/2000, recommended starting dilution is 1/1000.
  Flow Cytometry.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant human α-Crystallin B (1-175 aa) purified from E. coli
• Specificity: The antibody recognizes Human and Mouse Alpha-Crystallin B.
  Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: crystallin alpha B
• Database Link:
  Entrez Gene 1410 Human
  UniProt ID P02511
• Background: α-Crystallins are composed of two gene products; α-Crystallin A and B, for acidic and basic, respectively. α-Crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20). They act as molecular chaperones and hold them in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the α-Crystallin A and B subunits have a 3:1 ratio, respectively. Two additional functions of α-crystallins are an autokinase activity and participation in the intracellular architecture. α-Crystallin B is expressed widely in many tissues and organs and occurs in many neurological diseases.
• Synonyms: Alpha(B)-crystallin, CRYAB, Heat shock protein beta-5, Renal carcinoma antigen NY-REN-27, HspB5