CEBP Beta (CEBPB) Mouse Monoclonal Antibody [Clone ID: 47A1]

CAT#: SM6009S

CEBP Beta (CEBPB) mouse monoclonal antibody, clone 47A1, Purified

Product Images

Western blot analysis: NIH 3T3 cell lysates (30 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with SM6009 CEBPB antibody (1/1,000). Protein was visualized using a Goat anti-Mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Immunohistochemistry: CEBPB antibody staining of Formalin-Fixed, Paraffin-Embedded Human Skin at 10 ug/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen.

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  Immunohistochemistry: CEBPB antibody staining of Formalin-Fixed, Paraffin-Embedded Human Tonsil at 10 ug/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen.

Specifications

Product Data

• Clone Name: 47A1
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA.
  Western blot (1/500-1/2,000)
  Recommended Starting Dilution: 1/1,000.
  Immunofluorescence.
  Immunohistochemistry on Paraffin Sections (10 µg/ml).
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Human C/EBP-beta (1-271 aa) purified from E. coli.
• Specificity: The antibody recognizes Human and Mouse C/EBP-beta.
  Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 1051 Human
  UniProt ID P17676
• Background: CCAAT/enhancer-binding proteins (C/EBPs) are a family of basic leucine zipper (bZIP) DNA binding proteins that play important roles in normal cellular differentiation and function in a variety of tissues. The C/EBP family is critical for innate immunity and inflammatory response, and consists of five core members: C/EBPα, C/EBPβ, C/EBPδ, C/EBPε, and C/EBPγ. Among these proteins, C/EBPβ is usually highly expressed in liver, intestine, lung and adipose. C/EBPβ has three isoforms, two activator, (LAP* and LAP) and a repressor (LIP). These isoforms are differentially translated from the same mRNA. The expression of these isoforms is developmentally and hormonally regulated.
• Synonyms: C/EBP beta, TCF5, LAP, NF-IL6