T Cell Receptor (TCR) alpha/beta Mouse Monoclonal Antibody [Clone ID: R73]

CAT#: SM321P

T Cell Receptor (TCR) alpha/beta mouse monoclonal antibody, clone R73, Purified

Specifications

Product Data

• Clone Name: R73
• Applications: FC, IHC, IP
• Recommended Dilution: Flow Cytometry: 1/50 - 1/100; Use 10µl of the suggested working dilution to label 100µl of whole blood or 10e6 cells in 100µl.
  Immunohistochemistry on frozen sections: 1/10 - 1/100.
  Immunoprecipitation.
• Reactivities: Monkey, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Rat T Blasts and Erythrocytes. Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line.
• Specificity: This antibody recognises a constant determinant on the beta chain of the alpha/beta T cell receptor, expressed by 97% of peripheral T cells as defined by the OX-52 marker. R73 is mitogenic for unseparated spleen cells and for purified T cells. In the rat thymus, mature medullary cells express the R73 determinant at the same levels as peripheral T cells, whereas 94% of CD4 - CD8 - thymocytes are R73 negative. This clone is reported to stimulate adhesion between Thymic Dendritic Cells and Thymocytes.
• Formulation: PBS, pH7.2 containing 0.09% Sodium Azide
  State: Purified
  State: Liquid purified IgG
• Concentration: lot specific
• Purification: Affinity chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Synonyms: TCRA, TCRB, T-Cell Receptor alpha, T-Cell Receptor beta, T-Cell Receptor alpha beta