T Cell Receptor (TCR) alpha/beta Mouse Monoclonal Antibody [Clone ID: R73]

CAT#: SM321LE

T Cell Receptor (TCR) alpha/beta mouse monoclonal antibody, clone R73, Low Endotoxin

Product Images

Staining of rat spleen cells with Mouse anti Rat TCR alpha/beta Low Endotoxin (Cat.-No SM321LE).

Specifications

Product Data

• Clone Name: R73
• Applications: FC, FN, IHC, IP
• Recommended Dilution: Functional Assays.
  Immunoprecipitation.
  Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 100 µl of whole blood or 10⁶ cells in 100 µl.
  Immunohistochemistry on Frozen Sections: 1/10-1/100.
• Reactivities: Monkey, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Rat T Blasts and Erythrocytes.
  Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line.
• Specificity: This antibody recognizes a constant determinant on the beta chain of the Alpha/Beta T Cell Receptor, expressed by 97% of peripheral T cells as defined by the OX-52 marker.
  Clone R73 is mitogenic for unseparated spleen cells and for purified T cells. In the rat thymus, mature medullary cells express the R73 determinant at the same levels as peripheral T cells, whereas 94% of CD4 - CD8 - thymocytes are R73 negative.
  This Clone is reported to stimulate adhesion between Thymic Dendritic Cells and Thymocytes (Colic et al. 2010).
• Formulation: PBS, pH 7.4
  State: Low Endotoxin
  State: Liquid purified IgG fraction from Tissue Culture Supernatant
  Stabilizer: None
  Preservative: None
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Synonyms: TCRA, TCRB, T-Cell Receptor alpha, T-Cell Receptor beta, T-Cell Receptor alpha beta