CD14 Mouse Monoclonal Antibody [Clone ID: MEM-15]

CAT#: SM3163P

CD14 mouse monoclonal antibody, clone MEM-15, Purified

Product Images

Surface staining of human peripheral blood cells with anti-human CD14 (MEM-15) PE.

Specifications

Product Data

• Clone Name: MEM-15
• Applications: FC, IP
• Recommended Dilution: Flow cytometry (4 μg/ml).
  Immunoprecipitation.
• Reactivities: Human, Primate
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria
• Specificity: The antibody reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages.
  The antibody also reacts with soluble forms of CD14 found in serum and in the urine of some nephrotic patients.
• Formulation: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  State: Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein A affinity chromatography; purity: > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: CD14 molecule
• Database Link:
  Entrez Gene 929 Human
  UniProt ID P08571
• Background: CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor; it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.
• Synonyms: CD14