CD14 Mouse Monoclonal Antibody [Clone ID: MEM-15]

CAT#: SM3163F

CD14 mouse monoclonal antibody, clone MEM-15, FITC

Product Images

Surface staining of human peripheral blood cells with anti-human CD14 (MEM-15) FITC.

Specifications

Product Data

• Clone Name: MEM-15
• Applications: FC
• Recommended Dilution: Flow cytometry (20 µl to label 10⁶ cells or 100 µl whole blood).
  The content of a vial (2 ml) is sufficient for 100 tests.
• Reactivities: Human, Primate
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria
• Specificity: The antibody MEM-15 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages. The antibody also reacts with soluble forms of CD14 found in serum and in the urine of some nephrotic patients.
• Formulation: Phosphate buffered saline (PBS) solution containing 15mM sodium azide
  Label: FITC
  State: Liquid purified Ig fraction
  Label: Conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.
• Conjugation: FITC
• Storage: Store the antibody at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD14 molecule
• Database Link:
  Entrez Gene 929 Human
  UniProt ID P08571
• Background: CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor; it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.
• Synonyms: CD14