Vimentin (VIM) Mouse Monoclonal Antibody [Clone ID: VI-01]
Mouse Monoclonal Anti-VIM Antibody, Purified
Product Datasheet for 'SM3128P'
|Applications||IF, IP, WB|
|Recommend Dilution||ICC/IF, WB|
|Immunogen||Pellet of pig brain cold stable proteins after depolymerization of microtubules|
|Formulation||PBS , pH~7.4|
|Predicted Protein Size||53.5 kDa|
|Background||Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types - fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. In non-vascular smooth muscle cellsand striated muscle, vimentin is often replaced by desmin, however, during regeneration, vimentin is reexpressed. Cells of the lymfo-haemopoietic system (lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts.Vimentin is also found in mesoderm derived epithelia, e.g. kidney (Bowman capsule), endometrium and ovary (surface epithelium), in myoepithelial cells (breast, salivary and sweat glands), an in thyroid gland epithelium. In these cell types, as in mesothelial cells, vimentin is coexpressed with cytokeratin.Furthermore, vimentin is detected in many cells from the neural crest. Particularly melanocytes express abundant vimentin. In glial cells vimentin is coexpressed with Glial Fibrillary Acidic Protein (GFAP).Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive. Mesoderm derived carcinomas like renal cell carcinoma, adrenal cortical carcinoma and adenocarcinomas from endometrium and ovary usually express vimentin. Also thyroid carcinomas are vimentin positive. Any low differentiated carcinoma may express some vimentin.Vimentin is frequently included in the so-called primary panel (together with CD45, cytokeratin, and S-100 protein). Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is strongly suggestive of a mesenchymal tumour or malignant melanoma.|
|Protein Families||ES Cell Differentiation/IPS|
|The use of this Antibodies has been cited in the following citations:|
Molecular mechanisms of improved adhesion and growth of an endothelial cell line cultured on polystyrene implanted with fluorine ions.
,Bacakova L, Mares V, Lisa V, Svorcmk V,
,PubMed ID 10817270
Fluorine ion-implanted polystyrene improves growth and viability of vascular smooth muscle cells in culture.
,Bacakova L, Mares V, Bottone MG, Pellicciari C, Lisa V, Svorcmk V,
J. Biomed. Mater. Res. 2000
,PubMed ID 10602070
A common antigenic determinant of vimentin and desmin defined by monoclonal antibody.
,Draberova E, Draber P, Havlmcek F, Viklick} V,
Folia Biol. (Praha) 1989
,PubMed ID 2465190
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.