MHC Class I (RT1Aa) Mouse Monoclonal Antibody [Clone ID: OX-18]
$490.00
5 Days*
Specifications
| Product Data | |
| Clone Name | OX-18 |
|---|---|
| Application | FC, IHC, IP |
| Recommended Dilution | Flow cytometry: use 10 µl of 1/50 - 1/100 diluted antibody to label 10e6 cells in 100 µl. (This product is routinely tested in flow cytometry on rat splenocytes). Immunohistochemistry on frozen sections: Acetone fixation is recommended - the antigen is sensitive to fixation with paraformaldehyde. Not suitable for immunohistochemistry on paraffin sections. ELISA. Immunoprecipitation. |
| Reactivity | Rat |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Rat spleen glycoproteins |
| Specificity | This antibody recognizes a monomorphic determinant of rat MHC Class I (RT1A), expressed by all rat strains. However, qualitative measurements suggest that not all of the class I molecules are recognized. |
| Buffer | PBS, pH 7.4, containing 0.09% sodium azide State: Purified State: Liquid IgG fraction |
| Concentration | lot specific |
| Purification | Protein G affinity chromatography |
| Conjugation | Unconjugated |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Database Link | |
| Background | MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen. |
| Note | MRC OX-18 has been used in immunoaffinity purification of rat MHC class I molecules. |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.