alpha 1 Fetoprotein (AFP) Mouse Monoclonal Antibody [Clone ID: AFP-11]

CAT#: SM3090P

alpha 1 Fetoprotein (AFP) mouse monoclonal antibody, clone AFP-11, Purified

Product Images

Immunohistochemistry staining of hepatocellular carcinoma (fetal liver; paraffin-embedded sections) with anti-human alpha-Fetoprotein (AFP-11).

Specifications

Product Data

• Clone Name: AFP-11
• Applications: ELISA, IHC, R, WB
• Recommended Dilution: Western Blot: 2 μg/ml, non-reducing conditions.
  Immunohistochemistry on paraffin sections: 10 µg/ml; Application note:heat mediated antigen retrieval (sodium citrate buffer); mAb incubation 1 hour / RT; detection DAB.
  Positive control: hepatocellular carcinoma.
  ELISA: The antibody AFP-11 has been tested as the capture antibody in a sandwich ELISA for analysis of human alpha-Fetoprotein in combination with antibody clone AFP-01 (cat. no. SM3089P/PX).
  RadioImmunoAssay.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified alpha-Fetoprotein
• Specificity: The antibody detects Alpha-Fetoprotein (AFP), an oncofetal antigen of 70 kDa.
• Formulation: PBS, pH 7.4 containing 15 mM sodium azide as preservative
  State: Purified
  State: Liquid purified immunoglobulin fraction
• Concentration: lot specific
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 174 Human
  UniProt ID P02771
• Background: AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.
• Synonyms: Alpha-1-fetoprotein, Alpha-fetoglobulin, HPAFP