CD63 Mouse Monoclonal Antibody [Clone ID: MEM-259]

CAT#: SM3035P

CD63 mouse monoclonal antibody, clone MEM-259, Purified

Product Images

Immunofluorescence staining of human skin fibroblasts with anti-CD63 (MEM-259; green) after co-incubation of living cells with human Transferrin - Dyomics 547 (red); cell nuclei stained with DAPI (blue).

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  Immunofluorescence staining of CD63 in human primary fibroblasts using anti-CD63 (MEM-259; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

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  Immunofluorescence staining of CD63 in human HeLa cell line using anti-CD63 (MEM-259; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

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  Flow cytometry analysis of IgE-activated peripheral blood stained with anti-human CD63 (MEM-259) purified, GAM-APC.

Specifications

Product Data

• Clone Name: MEM-259
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: Flow Cytometry: 2 µg/ml (Detection of activated Platelets, Neutrophils and Basophils).
  Immunoprecipitation.
  Western blot (Non-Reducing Conditions).
  Immunocytochemistry.
  Immunohistochemistry on Paraffin Sections: 10 µg/ml.
  Positive tissue: Spleen.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: HPB-ALL T cell line
• Specificity: The antibody reacts with CD63 (LAMP-3), a 40-60 kDa tetraspan glycoprotein expressed by granulocytes, platelets, T cells, monocytes/macrophages and endothelial cells. Cell surface exposition of CD63 is usually activation-dependent.
• Formulation: PBS, pH~7.4
  State: Purified
  State: Liquid purified Ig fraction from hybridoma culture supernatant (> 95% pure by SDS-PAGE)
  Preservative: 15 mM Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: CD63 molecule
• Database Link:
  Entrez Gene 967 Human
  UniProt ID P08962
• Background: CD63, a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. It is also present in the plasma membrane, usually following cell activation. Hence, it has become an widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas.
• Synonyms: OMA81H, Granulophysin, Tetraspanin-30, MLA1, TSPAN30, ME491