IL2 Receptor alpha (IL2RA) Mouse Monoclonal Antibody [Clone ID: MEM-181]

CAT#: SM3015PP

IL2 Receptor alpha (IL2RA) mouse monoclonal antibody, clone MEM-181, PerCP

Product Images

Surface staining of human PBMC with anti-human CD25 FITC (Clone MEM-181, SM3015F).The mononuclear cells were isolated from human peripheral blood, devided in aliquots for duplicate analysis and stimulated with PHA for 2 days. Panel A, C ?? staining with anti-human CD25 (Clone MEM-181)Panel B, D ?? staining with a standard anti-CD25 monoclonal antibody

Specifications

Product Data

• Clone Name: MEM-181
• Applications: FC
• Recommended Dilution: Flow Cytometry analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension.
  The content of a vial (1 ml) is sufficient for 100 tests.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: PHA-activated peripheral blood leucocytes
• Specificity: This antibody reacts with CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.
• Formulation: Phosphate buffered saline (PBS)
  Label: PerCP
  State: Liquid Ig fraction
  Preservative: 15 mM sodium azide
  Label: Conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
• Conjugation: PerCP
• Storage: Store undiluted at 2-8°C. DO NOT FREEZE! This products is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 3559 Human
  UniProt ID P01589
• Background: CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. Humanized anti CD25 antibodies represent a useful tool to reduce the incidence of allograft rejection as well as the severity of graft versus host reaction, and radioimmunoconjugates of anti-CD25 antibodies can be used against CD25 expressing lymphomas.
• Synonyms: Interleukin-2 receptor alpha chain, IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, p55, TAC antigen