CD16 (FCGR3A) Mouse Monoclonal Antibody [Clone ID: MEM-154]

CAT#: SM3011P

CD16 (FCGR3A) mouse monoclonal antibody, clone MEM-154, Purified

Product Images

Flow Cytometry: Surface staining of human peripheral blood cells with anti-Human CD16 antibody (Clone MEM-154) PE. The antibody MEM-154 does not react with CD16a present on NK cells in many subjects.

Specifications

Product Data

• Clone Name: MEM-154
• Applications: FC, FN, IP, WB
• Recommended Dilution: Flow Cytometry: 5-10 μg/ml.
  Positive control: PBL (peripheral blood lymphocytes).
  Immunoprecipitation.
  Western Blotting (Non-reducing conditions).
  Functional Assay (The antibody blocks binding of Human IgG to FcgammaRIII).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human granulocytes.
• Specificity: The antibody reacts with with the epitope on CD16 antigen that residing in proximity to FG loop (probably BC or C'E loop).
  The antibody MEM-154 recognizes a polymorphism of CD16a (NK cells form), it reacts well with granulocyte form (CD16b) of all subjects.
• Formulation: TBS, pH~8.0 containing 15 mM Sodium Azide as preservative.
  State: Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein-A
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Fc fragment of IgG receptor IIIa
• Database Link:
  Entrez Gene 2214 Human
  UniProt ID P08637
• Background: CD16 (FcgRIII) is a 50-80 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgRIII is expressed in two forms FcgRIII-A and -B. FcgRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FceRI-g subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgRIII-A is associated, moreover, with FceRI-b subunit. Besides IgG, FceRI-A can be triggered also by oligomeric IgE. FcgRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.
• Synonyms: FCGR3A, CD16A, FCG3, FCGR3, IGFR3, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcR-10, IgG Fc receptor III-2