Bromodeoxyuridine / BrDU Mouse Monoclonal Antibody [Clone ID: MoBu-1]

CAT#: SM3005F

Bromodeoxyuridine / BrDU mouse monoclonal antibody, clone MoBu-1, FITC

Specifications

Product Data

• Clone Name: MoBu-1
• Applications: FC, IHC
• Recommended Dilution: Immunohistochemistry.
  Flow Cytometry (Use at 1-5 µg/10e6 cells).
  This antibody is also useful for detecting proliferating cells by Flow Cytometry or Immunofluorescence staining. The FITC conjugate is particularly useful in these applications as a secondary antibody and not necessary for visualization. Also, a specific anti-FITC antibody conjugate can be used for signal amplification if desired.
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Bromodeoxyuridine conjugated to Helix Pomatia Haemocyanin used to immunize BALB/c mice.
• Specificity: Reacts with cells containing incorporated BrdU, showing a clear, nucleus confined, speckled pattern.
• Formulation: PBS containing 0.08% Sodium Azide as a preservative.
  Label: FITC
  State: Liquid purified IgG fration.
• Concentration: lot specific
• Conjugation: FITC
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Background: Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. Previous methods of detecting DNA included the use of [3H]-thymidine which would be incorporated into the DNA and could then the DNA could be quantified by autoradiography or scintillation counting. These methods are more difficult and require more cleanup due to the radioactive material. An immunohistochemical assay provides a much simpler and cleaner method for detecting DNA in cells.