Ptprc (CD45RA) Mouse Monoclonal Antibody [Clone ID: OX-33]

CAT#: SM279PX

Ptprc (CD45RA) mouse monoclonal antibody, clone OX-33, Purified

Specifications

Product Data

• Clone Name: OX-33
• Applications: FC, IHC
• Recommended Dilution: Flow Cytometry: 1/50 - 1/100; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
  Immunohistochemistry on frozen and paraffin sections: This clone has been described reacting with paraffin-embedded material following PLP fixation (periodatelysine-
  paraformaldehyde) - refer to ref. Whiteland, J.L. et al. (1995) - reference 4.
• Reactivities: Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified spleen leucocyte common antigen. Spleen cells from immunised BALB/c mice were fused with cells of the NSO/U myeloma cell line.
• Specificity: This antibody is directed against a high molecular weight band of the leucocyte common antigen (LCA). MRC OX-33 only labels B-cells among thoracic duct lymphocytes with little labelling in bone marrow and none on thymocytes.
• Formulation: PBS, pH7.2 containing 0.09% Sodium Azide
  State: Purified
  State: Liquid purified IgG
• Concentration: lot specific
• Purification: Affinity chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 24699 Rat
  UniProt ID P04157
• Background: CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200