CD43 / Leukosialin Mouse Monoclonal Antibody [Clone ID: W3/13HLK]
CD43 / Leukosialin mouse monoclonal antibody, clone W3/13HLK, Purified
|Recommended Dilution||Flow Cytometry.
Tissue Distribution by Flow Cytometry Analysis:
Rat Strain: Wister Rat
Cell Concentration: 1x10e6 cells per test
Antibody Concentration Used: 0.5μg/10e6 cells
Secondary Antibody: FITC Goat Anti-Mouse IgG
Percentage of cells stained above control:
Lymph Node 58.9%
|Immunogen||Rat thymocyte membrane
Immunocyte Donor: BALB/c spleen
Fusion Partner: NS1/1
|Specificity||Anti-rat CD43 monoclonal antibody recognizes a monomorphic determinant expressed on rat thymocytes, polymorphonuclear cells, plasma cells and stem cells, but not B lymphocytes or pre-B cells (1,3). The antigen is a heavily glycosylated glycoprotein of apparent molecular weight 95 kDa and has a high content of Olinked carbohydrate structures (3). This major glycoprotein of thymocytes and T lymphocytes is referred to by several names including leukocyte sialoglycoprotein and leukosialin. The carbohydrate structures of leukosialin account for approximately 60% of its weight (2). On thymocytes, this glycoprotein is the main target for binding of peanut lectin (4). This antibody is useful for labelling T but not B lymphocytes and in studies on stem cells since pre-B cells are not labelled while the multipotential stem cell is. It may also be used in analysis of NK cells (5) and in molecular studies of the sialoglycoprotein which it recognizes.|
|Formulation||PBS containing 0.02% sodium azide (NaN3) as preservative
State: Liquid purified Ig fraction
|Purification||Affinity chromatography on Protein G|
|Storage||Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Synonyms||Leukocyte sialoglycoprotein, Sialophorin, Galactoglycoprotein, SPN|
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.