CD43 / Leukosialin Mouse Monoclonal Antibody [Clone ID: W3/13HLK]
CD43 / Leukosialin mouse monoclonal antibody, clone W3/13HLK, FITC
|Recommended Dilution||Flow Cytometry.
Tissue Distribution by Flow Cytometry Analysis:
Rat Strain: Wister Rat.
Cell Concentration: 1x10e6 cells per test.
Antibody Concentration Used: 0.5 μg/106 cells.
Secondary Antibody: FITC Goat Anti-Mouse IgG.
Cell Source: Percentage of cells stained above control
Lymph Node: 58.9%
|Immunogen||Rat thymocyte membrane preparation.|
|Specificity||This CD43 monoclonal antibody recognizes a monomorphic determinant expressed on Rat thymocytes, polymorphonuclear cells, plasma cells and stem cells, but not B lymphocytes or pre-B cells (1,3).
This antibody is useful for labelling T but not B lymphocytes and in studies on stem cells since pre-B cells are not labelled while the multipotential stem cell is. It may also be used in analysis of NK cells (5) and in molecular studies of the sialoglycoprotein which it recognizes.
|Formulation||PBS containing 0.02% Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
State: Liquid purified IgG fraction
Label: Fluorescein Isothiocyanate Isomer 1
|Storage||Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
This product is photosensitive and should be protected from light.
|Stability||Shelf life: one year from despatch.|
|Background||The antigen is a heavily glycosylated glycoprotein of apparent molecular weight 95 kDa and has a high content of Olinked carbohydrate structures (3). This major glycoprotein of thymocytes and T lymphocytes is referred to by several names including leukocyte sialoglycoprotein and leukosialin. The carbohydrate structures of leukosialin account for approximately 60% of its weight (2). On thymocytes, this glycoprotein is the main target for binding of peanut lectin (4).|
|Synonyms||Leukocyte sialoglycoprotein, Sialophorin, Galactoglycoprotein, SPN|
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.