Lag3 Rat Monoclonal Antibody [Clone ID: C9B7W]

CAT#: SM2270PS

Lag3 rat monoclonal antibody, clone C9B7W, Purified

Product Images

Staining of CD223 transfected cells with RAT ANTI MOUSE CD223

Specifications

Product Data

• Clone Name: C9B7W
• Applications: FC
• Recommended Dilution: Flow Cytometry: 1/50 - 1/200; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Murine CD223 Ig fusion protein. Cells from immunised Lewis rats were fused with cells of the Sp/20 myeloma cell line.
• Specificity: This antibody recognises lymphocyte activation gene-3 (LAG-3), that is also referred to as CD223. Clone C9B7W recognises an epitope within the D2 domain of CD223. The antibody is reported to block the in vitro function of murine LAG-3 but studies suggest that the antibody does not block binding of LAG-3 to MHC class II. We recommend the use of SM2268LE for this purpose.
• Formulation: PBS, pH7.4 containing 0.09% Sodium Azide
  State: Purified
  State: Liquid purified IgG
• Concentration: lot specific
• Purification: Affinity chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: lymphocyte-activation gene 3
• Database Link:
  Entrez Gene 16768 Mouse
  UniProt ID Q61790
• Background: CD223 is a 70kD activation-induced cell surface molecule. Murine CD223 is expressed on activated CD4 positive and CD8 positive alpha/beta T lymphocytes and a subset of natural killer (NK) cells. CD223 binds to MHC class II molecules with high affinity and is reported to negatively regulate T cell homeostasis and T cell expansion.
• Synonyms: LAG-3, FDC