Cd28 Mouse Monoclonal Antibody [Clone ID: E18]
$1,000.00
5 Days*
Specifications
| Product Data | |
| Clone Name | E18 |
|---|---|
| Application | FC, FN |
| Recommended Dilution | Functional Assays. Flow Cytometry: Use 10 µl of 1/50-1/100 diluted to label 106 cells in 100 µl. |
| Reactivity | Mouse |
| Antibody Host | Mouse |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Immunogen | Alternate immunisation with A20 cells expressing mCD28 and recombinant mCD28Ig. Spleen cells from immunised CD28-/-knockout mice were fused with cells of the X63Ag8.653 myeloma cell line. |
| Specificity | This antibody recognises CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. Clone E18 has been shown to be able to stimulate murine T lymphocytes in combination with anti-mouse CD3. |
| Buffer | PBS, pH 7.4 State: Low Endotoxin State: Liquid purified IgG fraction Stabilizer: None Preservative: None |
| Concentration | lot specific |
| Purification | Affinity chromatography on Protein G |
| Conjugation | Unconjugated |
| Storage | Store the antibody undiluted at -20°C. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | CD28 antigen |
| Database Link | |
| Background | Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95% of CD4+ cells and 50% of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells. The first is mediated via the T cell receptor following its interaction with antigenic peptide MHC complexes, and the second is delivered by accessory or costimulating molecules through their counter receptors on T lymphocytes. CD28 bears structural homology to CTLA 4 which is expressed at very low levels on the surface of CD4+ and CD8+ peripheral blood cells only following activation. CD28 is the natural receptor for the B7/BB 1 ligand (CD80) a 55-60 kDa glycoprotein which is expressed on activated B lymphocytes, on dendritic cells and on interferon gamma treated monocytes. The binding of B7 1/BB 1 molecules to CD28 is involved in T lymphocyte activation and in the initiation and maintenance of chronic inflammation. |
| Synonyms | TP44 |
| Note | Endotoxin Level: less than 0.01 EU/µg |
| Reference Data | |
| Protein Categories | CD Markers, Cytokines, Membrane Proteins |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.