Tubulin (TUBA1B) (Tyr-Tubulin) Rat Monoclonal Antibody [Clone ID: YL1/2]

CAT#: SM2202P

Tubulin (TUBA1B) (Tyr-Tubulin) rat monoclonal antibody, clone YL1/2, Purified

Product Images

HeLa whole cell lysate probed with Rat anti Tubulin Alpha antibody HRP conjugated

Specifications

Product Data

• Clone Name: YL1/2
• Applications: ELISA, IF, IHC, IP, R, WB
• Recommended Dilution: ELISA: 1/100-1/1000 (This antibody is routinely tested in ELISA on Tubulin) (3).
  Immunoprecipitation.
  Radioimmunoassay.
  Immunofluorescence.
  Western blotting: Suitable for use as a Loading Control.
  Immunohistochemistry on Frozen Sections.
• Reactivities: Mammalian, Yeast, Birds
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Yeast Tubulin.
  Spleen cells from immunised LOU rats were fused with cells of the Y3.Ag.1.2.3 rat myeloma cell line.
• Specificity: This antibody SM2202P recognizes the alpha subunit of Tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin) (2). The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin).
  The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized Actin (5).
• Formulation: PBS
  State: Purified
  State: Liquid purfied IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G from tissue culture supernatant
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: tubulin alpha 1b
• Database Link:
  Entrez Gene 10376 Human
  UniProt ID P68363
• Background: Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin.
• Synonyms: Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Gap junction, Pathogenic Escherichia coli infection