ICAM2 Rat Monoclonal Antibody [Clone ID: mIC2/4 (3C4)]
Specifications
| Product Data | |
| Clone Name | mIC2/4 (3C4) |
|---|---|
| Application | FC, FN, IP |
| Recommended Dilution | Flow Cytometry: 1/50 - 1/200; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl. Immunoprecipitation. Functional Assays. |
| Reactivity | Mouse |
| Antibody Host | Rat |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Immunogen | COS cells transfected with murine ICAM-2. Spleen cells from immunised Lewis rats were fused with cells of the Mouse P3X63Ag8.653 myeloma cell line. |
| Specificity | This antibody recognises intercellular adhesion molecule-2 (ICAM-2), also known as CD102. Clone mIC2/4 is reported to inhibit interactions between CD102 and LFA-1. |
| Buffer | PBS, pH7.4 State: Low Endotoxin State: Liquid purified IgG |
| Concentration | lot specific |
| Purification | Affinity chromatography on Protein G |
| Conjugation | Unconjugated |
| Storage | Store the antibody at -20°C. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | intercellular adhesion molecule 2 |
| Database Link | |
| Background | Murine CD102 is a glycosylated cell surface protein that is broadly expressed on most leucocytes and is constitutively expressed on endothelial cells. CD102 interacts with the CD11a/CD18 integrin LFA-1. Reports suggest that CD102 may play a role in lymphocyte re-circulation and T-cell activation. |
| Synonyms | ICAM-2 |
| Note | Endotoxin Level: less than 0.01 EU/µg |
| Reference Data | |
| Protein Categories | CD Markers, Intracellular Proteins, Membrane Proteins |
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