CD16/CD32 Rat Monoclonal Antibody [Clone ID: FCR 4G8]

CAT#: SM2093LE

CD16/CD32 rat monoclonal antibody, clone FCR 4G8, Low Endotoxin

Specifications

Product Data

• Clone Name: FCR 4G8
• Applications: FC
• Recommended Dilution: Flow Cytometry: 1/25 - 1/50; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
  Functional Assays.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: PU5 1.8 IOE7 Balb/c mouse cell line. Cells from immunised rats were fused with cells of the mouse P3X63-Ag8.653 myeloma cell line.
• Specificity: This antibody recognises an epitope expressed by the low affinity Fc receptors, Fc gamma III (CD16) and Fc gamma II (CD32).
• Formulation: PBS, pH7.4
  State: Low Endotoxin
  State: Liquid purified IgG
• Concentration: lot specific
• Purification: Affinity chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: In the mouse only the transmembrane form of CD16 is reported to exist, which is expressed on macrophages, NK cells, granulocytes, myeloid precursors, and subpopulations of T lymphocytes. CD32 is primarily expressed on cells of the myeloid lineage and also on mature B lymphocytes.
• Synonyms: IgG Fc receptor III-2, Fc-gamma RIII-alpha, Fc-gamma RIIIa, FcRIIIa, Fc-gamma RIII, FcRIII, CD16a, FcR mouse Seroblock, FCGR3A, FCG3, IGFR3, Fc gamma receptor IIB, Fc-gamma-RIIB, FcRII, Ly-17, Fcgr2
• Note: Endotoxin Level: less than 0.01Eu/µg