Clec7a Rat Monoclonal Antibody [Clone ID: 2A11]
Specifications
| Product Data | |
| Clone Name | 2A11 |
|---|---|
| Application | FC |
| Recommended Dilution | Flow Cytometry (Neat -1/10): Use 10 ul of the suggested working dilution to label 10e6 cells in 100 ul. |
| Reactivity | Mouse |
| Antibody Host | Rat |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Immunogen | Dectin-1 transfected NIH3T3 cells and recombinant soluble Dectin-1. Spleen cells from immunised Fischer rats were fused with cells of the rat Y3 myeloma cell line2 |
| Specificity | Recognises Murine Beta-Glucan Receptor (Dectin-1). This antibody inhibits the binding of zymosan to macrophages via the beta-glucan receptor. The low endotoxin version SM2025LE is recommended for Functional Studies. |
| Buffer | PBS containing 0.09% Sodium Azide as preservative and 1% BSA and 5% sucrose as stabilizers. Label: PE State: Lyophilized purified IgG fraction. Label: R. Phycoerythrin (RPE) |
| Reconstitution Method | Restore with 1 ml distilled water |
| Purification | Affinity Chromatography on Protein G. |
| Conjugation | PE |
| Storage | Store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | C-type lectin domain family 7, member a |
| Database Link | |
| Background | Dectin-1 is predominantly expressed by cells of the monocyte/macrophage and neutrophil lineages, but also at lower levels by dendritic cells and a subpopulation of T cells. As a major leucocyte receptor for beta-glucan this molecule may have a key role in the immunomodulatory effects of beta-glucans and in the host response to fungal pathogens. Recent work has shown that Dectin-1 may stimulate reactive oxygen production in macropahges via the protein tyrosine kinase known as Syk. |
| Synonyms | Beta-glucan receptor, BGR, CLECSF12, DECTIN1 |
| Reference Data | |
| Protein Categories | CD Markers, Cytokines, Immune system diseases, Intracellular Proteins, Membrane Proteins |
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