IGF2R Mouse Monoclonal Antibody [Clone ID: MEM-238]

CAT#: SM2004PS

IGF2R mouse monoclonal antibody, clone MEM-238, Purified

Product Images

Western Blotting analysis (non-reducing conditions) of CD222 in whole cell lysate of JURKAT human peripheral blood T cell leukemia cell line. Lane 1: immunostaining with anti-CD222 (MEM-238) Lane 2: immunostaining with Isotype mouse IgG1 control

Specifications

Product Data

• Clone Name: MEM-238
• Applications: FC, IP, WB
• Recommended Dilution: Flow cytometry: 1-2 µg/ml.
  Immunoprecipitation.
  Western blot (non reducing conditions).
• Reactivities: Human, Mouse, Primate
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Vaccinia virus encoding CD222
• Specificity: The antibody recognizes an epitope between domains 2 and 5 of CD222 (IGF2 receptor), a ubiquitously expressed 250 kDa multifunctional type I transmembrane protein.
  The majority of CD222 is found in the late endosomal/prelysosomal compartment, 5-10% in the plasma membrane and the truncated (220 kDa) form of CD222 is present in human and bovine serum.
• Formulation: PBS, pH~7.4 containing 15 mM Sodium Azide as preservative
  State: Purified
  State: Liquid purified Ig fraction (> 95% by SDS-PAGE)
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein-A
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: insulin like growth factor 2 receptor
• Database Link:
  Entrez Gene 3482 Human
  UniProt ID P11717
• Background: CD222 is a ubiquitously expressed 250 kDa transmembrane protein. No more than 10% of CD222 is present on the cell surface where it serves as a multifunctional receptor. Intracellular (major) fraction of CD222 is involved in transport of newly synthesized lysosomal enzymes modified by mannose 6-phosphate from Golgi apparatus to lysosomes. The cell surface CD222 binds and internalizes exogeneous mannose 6-phosphate-containing ligands. Importantly, CD222 is crutial for internalization and degradation of insulin-like growth factor 2, thus controling cell growth. CD222 also complexes CD87 (urokinase-type plasminogen-activator receptor), plasminogen and latent TGF-b, last but not least CD222 serves as a receptor for heparanase and even for Listeria.
• Synonyms: CI-MPR, Insulin-like growth factor 2 receptor, Insulin-like growth factor II receptor, M6P/IGF2 receptor, CI Man-6-P receptor, M6PR, Late Endosome Marker, M6P/IGF2R, MPR 300