IGF2R Mouse Monoclonal Antibody [Clone ID: MEM-238]

CAT#: SM2004AC

IGF2R mouse monoclonal antibody, clone MEM-238, APC

Product Images

Western Blotting analysis (non-reducing conditions) of CD222 in whole cell lysate of JURKAT human peripheral blood T cell leukemia cell line. Lane 1: immunostaining with anti-CD222 (MEM-238) Lane 2: immunostaining with Isotype mouse IgG1 control (PPV-06)

Specifications

Product Data

• Clone Name: MEM-238
• Applications: FC, WB
• Recommended Dilution: Flow Cytometry analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10e6 cells in a suspension.
  The content of a vial (1 ml) is sufficient for 100 tests.
• Reactivities: Human, Primate
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Vaccinia virus encoding CD222
• Specificity: This antibody recognizes an epitope between domains 2 and 5 of CD222 (IGF2 receptor), a ubiquitously expressed 250 kDa multifunctional type I transmembrane protein. The majority of CD222 is found in the late endosomal/prelysosomal compartment, 5-10% in the plasma membrane and the truncated (220 kDa) form of CD222 is present in human and bovine serum.
• Formulation: Conjugated with cross-linked Allophycocyanin (APC) under optimum conditions
  Label: APC
  State: Liquid purified Ig fraction
  Label: Conjugated with cross-linked Allophycocyanin under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
• Conjugation: APC
• Storage: Store the antibody at 2 - 8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: insulin like growth factor 2 receptor
• Database Link:
  Entrez Gene 3482 Human
  UniProt ID P11717
• Background: CD222 (CIMPR, cation-independent mannose 6-phosphate receptor; IGF2 receptor) is a ubiquitously expressed 250 kDa transmembrane protein. No more than 10% of CD222 is present on the cell surface where it serves as a multifunctional receptor. Intracellular (major) fraction of CD222 is involved in transport of newly synthesized lysosomal enzymes modified by mannose 6-phosphate from Golgi apparatus to lysosomes. The cell surface CD222 binds and internalizes exogeneous mannose 6-phosphate-containing ligands. Importantly, CD222 is crutial for internalization and degradation of insulin-like growth factor 2, thus controling cell growth. CD222 also complexes CD87 (urokinase-type plasminogen-activator receptor), plasminogen and latent TGF-beta, last but not least CD222 serves as a receptor for heparanase and even for Listeria.
• Synonyms: CI-MPR, Insulin-like growth factor 2 receptor, Insulin-like growth factor II receptor, M6P/IGF2 receptor, CI Man-6-P receptor, M6PR, Late Endosome Marker, M6P/IGF2R, MPR 300

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Lysosome