CD177 Mouse Monoclonal Antibody [Clone ID: MEM-166]

CAT#: SM2003S

CD177 mouse monoclonal antibody, clone MEM-166, Purified

Product Images

Surface staining of human peripheral blood cells with anti-CD177 (MEM-166) purified, GAM-APC.

Specifications

Product Data

• Clone Name: MEM-166
• Applications: FC, IP, WB
• Recommended Dilution: Western Blot (Non-Reducing Conditions).
  Immunoprecipitation.
  Flow Cytometry: 1 µg/ml.
• Reactivities: Human, Primate
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human Granulocytes
• Specificity: This antibody reacts with CD177 (Neutrophil specific antigen 1), a 60 kDa GPI-linked cell surface glycoprotein of uPAR family, expressed on granulocytes and in bone marrow early erythroblasts, megakaryocytes, promyelocytes and myelocytes.
• Formulation: PBS
  State: Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 15 mM Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: CD177 molecule
• Database Link:
  Entrez Gene 57126 Human
  UniProt ID Q8N6Q3
• Background: CD177 (NP1), a glycosyl phosphatidylinositol (GPI) linked N glycosylated cell surface glycoprotein, was first described in a case of neonatal alloimmune neutropenia and analyses showed cell surface expression of a 60 kDa protein, 14 kDa greater than the predicted size, probably due to the presence of 3 potential N glycosylation sites. Immunohistochemistry demonstrated expression in bone marrow early erythroblasts, megakaryocytes, promyelocytes, and myelocytes. The neutrophil NB1 antigen (CD177) is expressed by 97% of the caucasian population.
• Synonyms: NB1, PRV1, HNA-2a, HNA2a