Granzyme B (GZMB) Mouse Monoclonal Antibody [Clone ID: GB7]

CAT#: SM1804P

Granzyme B (GZMB) mouse monoclonal antibody, clone GB7, Purified

Specifications

Product Data

• Clone Name: GB7
• Applications: IHC, WB
• Recommended Dilution: Western blot.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
  Requires antigen retrieval using heat treatment with Sodium Citrate buffer pH 6.0.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant Granzyme B.
  Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line
• Specificity: This antibody recognizes the serine protease Granzyme B, which is important in the induction of apoptosis in target cells by cytolytic lymphocytes (CTLs).
• Formulation: TRIS buffered saline, pH 8.0
  State: Purified
  State: Liquid purified IgG fraction from Tissue Culture Supernatant
  Preservative: 0.01% Thiomersal
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: granzyme B
• Database Link:
  Entrez Gene 3002 Human
  UniProt ID P10144
• Background: Granzyme A and Granzyme B are serine proteases that mediate apoptotic signaling in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Both granzyme A and granzyme B are synthesized as inactive proenzymes, and they are stored within cytolytic granules and released by effector cells during degranulation. In activated CTLs, granzyme A and granzyme B are processed and activated by cathepsin C, and they then function to induce apoptosis by two distinct pathways.
  Granzyme B proteolytically cleaves and activates members of the caspase family of cysteine proteases, including caspase-3, caspase-6, caspase-7 and caspase-9. When cleaved, these caspases assemble into active holoenzymes that then mediate apoptosis through a defined proteolytic cascade involving nuclear lamins and PARP (poly ADP ribose polymerase).
  Granzyme A mediates the activation of apoptosis by inducing single-strand DNA breaks, membrane perturbation and nuclear condensations in an alternative pathway that is independent from caspase activation or the caspase proteolytic cascade.
• Synonyms: Granzyme-2, CGL1, CTSGL1, CSPB, CTLA1, CTLA-1, GRB, SECT, Fragmentin-2