CD147 (BSG) Mouse Monoclonal Antibody [Clone ID: MEM-M6/1]
Product Data | |
Clone Name | MEM-M6/1 |
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Application | FC, IF, IHC, IP, WB |
Recommended Dilution | Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 106 cells or 100 µl whole blood. Immunoprecipitation. Western Blot (under non-reducing conditions). Immunohistochemistry on Paraffin Sections: 10 µg/ml. This Basigin antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. |
Reactivity | Human |
Antibody Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | CD147Rg, consisting of the cDNA coding for the hinge region, CH2 and CH3 domains of Human IgG1. |
Specificity | This antibody recognises the CD147 cell surface antigen. |
Buffer | PBS, pH 7.4 State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide |
Concentration | lot specific |
Purification | Affinity Chromatography on Protein G |
Conjugation | Unconjugated |
Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Shipping | Blue Ice |
Gene Name | basigin (Ok blood group) |
Database Link | |
Background | CD147 is a 50-60kD glycoprotein also known as neurothelin and basigin. CD147 is broadly expressed on peripheral blood cells and by endothelial cells. Expression upon T cells is increased following activation. |
Synonyms | Basigin, BSG, EMMPRIN, TCSF |
Reference Data |
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