HLAG (HLA-G) Mouse Monoclonal Antibody [Clone ID: MEM-G/9]

CAT#: SM1592AS

HLAG (HLA-G) mouse monoclonal antibody, clone MEM-G/9, Azide Free

Product Images

Surface staining of HLA-G1 transfectants (LCL-HLA-G1) using anti-HLA-G (MEM-G/9) FITC.

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  Immunoprecipitation of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by anti-human HLA-G (MEM-G/9) and protein G. HLA-G was detected by anti-human HLA-G (4H84) and goat anti-mouse HRP in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2).

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  Double surface staining of HLA-G1 transfectants (viable cells gate) using anti-human HLA-G (MEM-G/9) APC and anti-human HLA-G (MEM-G/11) FITC.

Specifications

Product Data

• Clone Name: MEM-G/9
• Applications: ELISA, FC, IF, IHC, IP
• Recommended Dilution: Flow cytometry: Recommended dilution: 0.3-4 μg/ml; positive control: JEG-3 human choriocarcinoma cell line.
  Immunocytochemistry: Recommended dilution: 2-5 μg/ml.
  ELISA: The antibody MEM-G/9 has been tested as the capture antibody in a sandwich ELISA for analysis of human HLA-G in combination with antibody B2M-01 or with antibody W6/32. Coating antibody 10 μg/ml, detection antibody (biotin or peroxidase conjugate) 1 μg/ml.
  Immunohistochemistry: Recommended dilution: 1-4 μg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human HLA-G refolded with beta2-microglobulin and peptide
• Specificity: The antibody reacts with native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form.
• Formulation: Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized
  State: Azide Free
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Purified by protein A; purity: > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: major histocompatibility complex, class I, G
• Database Link:
  Entrez Gene 3135 Human
  UniProt ID P17693
• Background: HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.
• Synonyms: HLA-6.0, HLAG, MHC class I antigen G
• Note: The clone MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris, 2003).