HLAG (HLA-G) Mouse Monoclonal Antibody [Clone ID: MEM-G/1]

CAT#: SM1591S

HLAG (HLA-G) mouse monoclonal antibody, clone MEM-G/1, Purified

Product Images

Immunohistochemistry staining with anti-human HLA-G (MEM-G/1) - pulmonary disseases (paraffin-embedded sections). The antibody MEM-G/1 stains infiltrating macrophages in pulmonary diseases. In the top left corner see the detail of macrophage.

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  Immunohistochemistry staining with anti-human HLA-G (MEM-G/1) of first trimester placenta (paraffin-embedded sections).

Specifications

Product Data

• Clone Name: MEM-G/1
• Applications: IHC, WB
• Recommended Dilution: Western Blotting. Recommended dilution 1-2 μg/ml.
  Immunohistochemistry on Paraffin Sections: 1/60-1/100 Incubation Time: 1 hour at RT.
  Positive tissue: Human placenta - extravillous cytotrophoblast.
  Staining technique: Standard ABC technique.
  Pretreatment: Heat retrieval in 0.01 M citrate buffer (4x2 min. in microwave oven).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Human HLA-G denaturated heavy chain
• Specificity: The antibody reacts with denaturated HLA-G heavy chain.
  HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.
• Formulation: Phosphate buffered saline (PBS), pH~7.4 with 15 mM Sodium Azide
  State: Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein-A
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C. Do Not freeze.
• Stability: Shelf life: one year from despatch.
• Gene Name: major histocompatibility complex, class I, G
• Database Link:
  Entrez Gene 3135 Human
  UniProt ID P17693
• Background: HLA G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of a heavy chain (with a1, a2, a3 domains) and a light chain (beta 2 microglobulin). Alternate splicing provides both soluble and membrane bound forms of HLA G, with two soluble isoforms and four membrane bound isoforms. HLA G may be part of the fetal protection system and is expressed on fetal derived placental cells.
• Synonyms: HLA-6.0, HLAG, MHC class I antigen G