PCNA Mouse Monoclonal Antibody [Clone ID: PC10]

CAT#: SM1421P

PCNA mouse monoclonal antibody, clone PC10, Purified

Product Images

Western blot analysis of K562 erythroleukemic cell line whole cell lysate probed with Mouse anti Human PCNA antibody (cat.-No SM1421P) followed by HRP conjugated Goat anti Mouse IgG secondary antibody, visualized using chemiluminescence.

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  Western blot analysis of HeLa Nuclear Extract probed with Mouse anti Human PCNA (cat.-No SM1421P).

Specifications

Product Data

• Clone Name: PC10
• Applications: FC, IHC, IP, WB
• Recommended Dilution: Flow Cytometry: Use 10 µl of 1/100 diluted antibody to label 10e6 cells in 100 µl. Cell permeabilisation required).
  Immunohistochemistry on Frozen Sections and Paraffin embedded sections: 1/50-1/100. It does not require antigen unmasking.
  Recommended Positive Control Tissue: Tonsil.
  Immunoprecipitation.
  Western blot (1/1000).
• Reactivities: Amphibian, Drosophila, Fish, Mammalian, Yeast
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Rat PCNA made in the protein A expression vector pR12T2.
• Specificity: This antibody recognises PCNA, a nuclear protein vital for cellular DNA synthesis.
• Formulation: PBS, pH 7.4, containing 0.09% Sodium Azide as preservative.
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: proliferating cell nuclear antigen
• Database Link:
  UniProt ID P12004
• Synonyms: Cyclin