alpha Defensin 1 (DEFA1) Mouse Monoclonal Antibody [Clone ID: DEF-3]

CAT#: SM1382

alpha Defensin 1 (DEFA1) mouse monoclonal antibody, clone DEF-3, Purified

Product Images

Defensin antibody staining of Human Tonsil Paraffin Section.

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  Defensin antibody staining of Human Tonsil Frozen Section.

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  Defensin antibody staining of Human Placenta Frozen Section.

Specifications

Product Data

• Clone Name: DEF-3
• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Frozen Sections: 0.2 µg/ml (1/1000).
  Immunohistochemistry on Paraffin Sections: 0.5 µg/ml (1/400), Proteinase K pretreatment for antigen retrieval is recommended.
  Positive Control: Human tonsil.
  Has been discribed to work in FACS with permeabilized cells.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Native defensins.
  The epitope has not been further characterized.
• Specificity: Several Defensins are recognized.
  This antibody recognises a family of Cyclic Peptides in Neutrophils.
  Human:
  Defensin 1-3 (HNP-1 to HNP-3) in Human Neutrophils.
  Synthetic Defensin-1 and -2 (Bachem, Bubendorf CH) stain also positively. Not tested with Defensin-4. A side reaction to elastin has been observed in humans.
  Other: not tested. Defensins have been described in rabbits (NP-1 to NP-5, NP3a = Corticostatin), and in mice (cryptodin).
• Formulation: PBS, pH 7.2, containing 5 mg/ml BSA and 0.09% sodium azide
  State: Purified
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore with 0.5 ml distilled water.
• Concentration: 0.2 mg/ml (after reconstitution)
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Store the original vial at 2-8°C.
  Freeze stock solution or aliquots thereof at -20°C.
  Avoid repeated freezing and thawing.
  Do not freeze working dilutions.
• Stability: Shelf life: one year from despatch.
• Gene Name: defensin alpha 1
• Database Link:
  Entrez Gene 728358 HumanEntrez Gene 1667 Human
  UniProt ID P59665
• Background: Defensins are a family of cyclic peptides containing 29-35 amino acids (MW < 3500) which tend to form aggregates. Four of these peptides are described in humans (HNP-1 to 4), six in rabbits (NP-1 to 5). Synonyms are MCP-1 for NP-1, MCP-2 for NP-2 and corticostatin for NP-3.
  Biological Functions: Various functions have been described for defensins. They are antibacterial, antifungal, chemotactic for monocytes, inhibitory for ACTH-induced corticosteroid synthesis and cytotoxic for cells. Defensins are inhibited by glucosaminoglycans (self protection for cells) and high concentrations of Ca2+ ions.
• Synonyms: DEFA1, DEF-A1, DEFA-1, DEF1, DEFA2, MRS, DEF-1, DEFA-2, DEF-A2, HNP-1, HP-1, HP1, HNP1, Neutrophil defensin 1, DEFA3, DEFA-3, DEF-A3, DEF3, DEF-3, HNP-3, HP-3, HP3, HNP3, Neutrophil defensin 3, Defensin alpha 1, Defensin alpha 3

Reference Data

• Protein Families: Druggable Genome