Fast skeletal muscle Troponin T Mouse Monoclonal Antibody [Clone ID: T1/61]

CAT#: SM1372P

Fast skeletal muscle Troponin T mouse monoclonal antibody, clone T1/61, Purified

Product Images

Immunohistochemistry: SM1372P/PT TNNT3 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Skeletal Muscle at 10 g/ml followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Clone Name: T1/61
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Western Blot.
  Immunohistochemistry on Frozen and Paraffin Sections: This product does not require protein digestion pre-treatment of paraffin sections; This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
  Recommended Positive Control Tissue: Skeletal muscle.
• Reactivities: Chicken, Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Troponin T, purified from chicken breast muscle.
  Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
• Specificity: This antibody recognises fast muscle Troponin T.
• Formulation: PBS containing 0.09% Sodium Azide as preservative.
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P12620
• Background: The contraction of striated muscle is initiated by the binding of intracellular calcium to the troponin complex located in the thin filaments. The complex consists of the 3 different troponins; troponin C, troponin T, and troponin I. Troponin T is the tropomyosin binding subunit of troponin which confers calcium sensitivity to striated muscle actomyosin ATPase activity. TNNT3 (Troponin T type 3 (skeletal, fast)) is one of 3 identified troponin T proteins which are encoded by different genes.
• Synonyms: TnTf, Beta TnTF, TNNT3